Table 1.
Binding Assay of Aptamer M3 to Different Cell Lines
| Cell Line/Aptamer | M3 |
|---|---|
| MDA-MB-231 | ++++ |
| MCF-7 | – |
| SK-BR-3 | – |
| BT474 | – |
| ZR-75-30 | + |
| MDA-MB-436 | + |
| LoVo | ++ |
| CL187 | – |
| SGC-7901 | ++ |
| BGC823 | + |
| A549 | – |
| C6 | + |
| HepG2 | + |
| HeLa | – |
| HEK293 | – |
| NIH 3T3 | – |
| CHO | – |
A threshold of the fluorescence intensity of flow cytometry analysis was set so that 95% of cells incubated with the FITC-labeled ssDNA library would have fluorescence intensity below it. The percentage of the cells with fluorescence above the set threshold was used to evaluate the binding capacity of the aptamer to the cells. Symbols: –, <14%; +, 15%–35%; ++, 36%–60%; ++++, >85%.