Figure 2.

Increased Delivery of LVs to Murine Liver by Hydrodynamic Tail-Vein Injections

(A) Bioluminescence imaging of mice administered with a vector dose of 4 μg p24 of pCCL-PGK-fLuc vector-encoding particles visualizes the localization and expression level of the luciferase reporter gene at different time points. Administration were as follows: conventional (0.4 mL) tail-vein injections (first row), “primed” injections (second row), hydrodynamic injections (third row), intrahepatic injections (fourth row), or an untreated mouse as control (fifth row). The mice were analyzed in an IVIS bioluminescence scanner after subcutaneous injections of luciferin for quantification of reporter gene activity. The colored scale indicates radiance (photons/s/cm²/steradian). (B) An ROI surrounding the liver was defined and radiance was quantified within each ROI. The mean and SD were calculated for each group (n = 6) and plotted at each sampled time point (3, 7, 14, 21, and 28 days post-injection). (C) Table showing the ratio of reporter activities in liver between indicated groups at days 14 and 28 and the associated p value in brackets below. One (*), two (**), or three (***) asterisks indicate significance levels below 0.05, 0.01, or 0.001, respectively.