Figure 8.
TGFβ1 reverses TNF-induced induction of Ccl20 mRNA. (A) YAMC colonocytes were treated with 10 ng/mL TNF or vehicle for 24 hours followed by the addition of 3 ng/mL TGFβ1 or vehicle for the indicated times followed by mRNA analysis. (B) IMCS4fl/fl cells were treated with TNF or vehicle for 24 hours followed by the addition of 3 ng/mL TGFβ1 and 100 ng/mL BMP2 for the indicated times. Colonoids were treated with vehicle or 100 ng/mL TNF for 24 hours followed by the addition of 3 ng/mL TGFβ1 for 8 hours. mRNA was quantified as described in Figure 7. For each group, n = 3 biological repeats (performed on 3 separate days). ∗P < .05, ∗∗P < .01. ve or veh, vehicle.