Figure 6.

Deletion of Gank does not affect the histologic response of mice to CCl₄-mediated liver injury. (A) H&E staining, (B) terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining, and (C) glutamine synthetase staining of livers of LoxP and Gank LKO mice at different time points after CCl₄ injection. The majority of glutamine synthetase staining is observed around central veins. Three animals per genotype per time point were analyzed with 5 fields examined per mouse. (D) Examination of baseline levels of CYP2E1 in livers of LoxP and Gank LKO mice by Western blot and by quantitative RT-PCR. P values were as follows: protein level (P > .2) and mRNA level (P > .4). Each sample on Western blot represented a biological replicate (5 LoxP mice and 7 Gank LKO mice), which also was used for quantitative RT-PCR. Both experiments had 2 technical replicates. (E) Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) from LoxP and Gank LKO mice at the 0-hour time point and at 48 hours after CCl₄ injection. Serum analysis was performed on 3 mice per genotype and per time point. P values for ALT were as follows: 0 hours (P > .99) and 48 hours (P < .0001). P values for AST were as follows: 0 hours (P > .99) and 48 hours (P < .0001). Two way analysis of variance. ∗∗∗∗P < .0001 LoxP vs Gank LKO.