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. 2018 Mar 8;67(3):349–359. doi: 10.1538/expanim.17-0137

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©2018 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 3. — CFU-F assay, sphere formation assay, and stemness markers. A, B: CFU-F frequency remained relatively higher in the SCB-MSC group than in the BMS-MSC group. The bar represents 1 cm in 3A. SCB-MSCs, subchondral bone-derived MSCs; BMS-MSCs, bone marrow suspension-derived MSCs. C, D: The results of primary and secondary sphere culture revealed a significant difference between the two groups. The bars represent 100 µm in 3C upper and 200 µm in 3C low, respectively. E: Comparison of mRNA expression of several stemness markers (Nanog, OCT4, and Sox-2) between two groups.