Skip to main content
. 2018 Mar 8;67(3):349–359. doi: 10.1538/expanim.17-0137

Table 1. Primer sequences.

genes primersequences Annealing temperature
HPRT forward 5′-GACCAGTCAACAGGGGACAT-3′ 60 °C
reverse 5′-ACACTTCGAGGGGTCCTTTT-3′
Runx2 forward 5′-ATTTCTCACCTCCTCAGCCC-3′
reverse 5′-TCCCAAGTTTCCCTCATCCC-3′
OPN forward 5′-TTTTGTCTCTTGGGCATGGC-3′
reverse 5′-GCATTCTGCGGTGTTAGGAG-3′
CEBP/α forward 5′-GGGACGCTAGGTGACAGAAT-3′
reverse 5′-GAAAGGACGCTGGCTGAAAA-3′
PPARγ forward 5′-TTGCTGTGGGGATGTCTCAT-3′
reverse 5′-TTTCCTGTCAAGATCGCCCT-3′
Sox9 forward 5′-ATGAAGATGACCGACGAGCA-3′
reverse 5′-ACTTGTCCTCTTCGCTCTCC-3′
Collage I forward 5′-CCAAGGGAGAGCAAGGAGAA-3′
reverse 5′-CCTTTGGGGCCTTCTTTTCC-3′
Nanog forward 5′-AAAACTCCCGACTCTGCAGA-3′
reverse 5′-AGGCTGGAGAGTTCTTGCAT-3′
4-Oct forward 5′-CGGAAGAGAAAGCGAACGAG-3′
reverse 5′-TGGCCTCAAAATCCTCTCGT-3′
Sox2 forward 5′-AAGGGAAATGGGGAGAGGTG-3′
reverse 5′-TGGATGGGATTGGTGGTCTC-3′