Table 4.

Comparison between novel PCR and other method.

References	Pretreatment			Reaction time	Discrimination on pathogenicity of V. P	Remarks
	Dye style	Reagent dosage	Treatment time
Bej et al., 1999	/	/	/	1 h 33 min	tlh, tdh, trh	It is a comprehensive detection of V. parahaemolyticus in selfish.
Garrido et al., 2012	/	/	/	55.5 min	tdh, trh1, trh2	It is a detection only for pathogenic V. parahaemolyticus.
Robert-Pillot et al., 2010	/	/	/	/	R72H, tdh, trh	Different reaction conditions were conducted on three target gene.
Eschbach et al., 2017	/	/	/	1 h 2 min	tlh, tdh, trh1, trh2	V. parahaemolyticus, V. cholerae and V. vulnifcus were only conducted in interlaboratory study.
Takahashi et al., 2005	/	/	/	1 h 8 min	toxR, tdh, trh	It has been employed in pure culture, seawater, and shellfish.
Zhang et al., 2015b	/	/	/	52 min	tlh	V. parahaemolyticus, L. monocytogenes, and Salmonella spp.
Panicker et al., 2004	/	/	/	1 h3 min	tlh, tdh, trh, ORF8	V. vulnificus, V. parahaemolyticus, and V. cholerae.
Lee and Levin, 2009	EMA	2.381 μM	15 min	1 h 35 min	/	V. vulnificus.
Zhang et al., 2015a	PMA	100 μM	30 min	52 min	tlh	V. parahaemolyticus and L. monocytogenes
This study	PMA	15 μM	20 min	34 min	tlh, tdh, ureR	The ureR was based on Nilsson and Turner (2016).