FIG 3 .

Both Gis2 and Znf9 bind the GGAUG element with specificity. (A) UV-cross-linked EMSA with either cell extract or recombinant Znf9 (rZnf9) (left) or recombinant Gis2 (rGis2) (right) with the TYE-705 RNA oligonucleotide alone or in the presence of 50× wild type or 50× mutant (mt) unlabeled competitor oligonucleotide. (B) UV cross-linked EMSA analysis of cell extracts of the wild type, gis2Δ mutant, znf9Δ mutant, and gis2Δ znf9Δ double mutant with the TYE705-labeled RNA oligonucleotide alone or in the presence of 50× wild type or 50× mutant unlabeled competitor.