FIG 6 .

Acceleration of RPL2 decay in response to temperature stress is unchanged by deletion of GIS2, ZNF9, or both genes. Analysis of RNA stability of RPL2 by Northern blot analysis in a time course experiment following the addition of 1,10-phenanthroline to halt transcription. Prior to membrane transfer, separated rRNA bands were visualized using SYBR safe nucleic acid gel stain, and the resulting intensity was used as a total RNA loading control. (A to D) The wild type (A), gis2Δ mutant (B), znf9Δ mutant (C), and the gis2Δ znf9Δ double mutant (D) were grown to mid-log phase at 30°C or shifted to prewarmed 37°C medium.