FIG 7 .

Deletion of GIS2 and ZNF9 affects basal RPL2 decay and polysome association. (A) Analysis of RNA stability at 30°C between the wild type and znf9Δ gis2Δ double mutant. RNA was isolated in a time course experiment after the addition of 1,10-phenanthroline to halt transcription. DKO, double knockout. (B) Analysis of polysome association of the RPL2 mRNA in the wild type or znf9Δ gis2Δ double mutant during mid-log growth at 30°C. Fractions were collected from the polysome profile gradients, and RNA was isolated and subjected to Northern blotting for RPL2.