FIG 8 .

GIS2 and ZNF9 are required for stress resistance and ergosterol biosynthesis. (A) Spot plate analysis of the wild type, gis2Δ mutant, znf9Δ mutant, and gis2Δ znf9Δ double mutant on YPD medium alone or with the indicated stressor. (B) Spot plate assay of the wild type, gis2Δ mutant, complemented gis2Δ mutant, and gis2 Δznf9Δ double mutant on YPD alone or with the indicated stressor. The stressors in panels A and B are antimycin A (Ant A), cobalt chloride (CoCl₂), fluconazole (FCZ), and peroxide (H₂O₂). (C) Analysis of ERG25 and RPL2 expression in response to cobalt chloride treatment in the wild type or gis2Δ znf9Δ double mutant. (D) Analysis of sterol content by heptane extraction in the wild type, gis2Δ mutant, znf9Δ mutant, and gis2Δ znf9Δ double mutant. Approximately 0.255 g (wet weight) (±0.005 g) of pelleted yeast cells were processed for sterol extraction for each strain. Data are representative of three biological replicates.