TABLE 1 .
DNA and RNA oligonucleotides used in this study
| Oligonucleotide name | Oligonucleotide sequencea |
|---|---|
| DNA oligonucleotides | |
| F-rZNF9-BglII | TAATAAAGATCTGATGTTTGGAGCTGCTGCTGTTCC |
| R-rZNF9-BglII | TAATAAAGATCTCAAGCACAGATACTATTACTCCGC |
| F-ZNF9upKO-XbaI | TAATAATCTAGAAGTAAGATCTTCTGCCCAGGCG |
| R-ZNF9upKO-BglII | TAATAAAGATCTGCCGTGTTCCTTCGTTGG |
| F-ZNF9downKO-MunI | TAATAACAATTGCATGACTCATCACTGACTGC |
| R-ZNF9downKO-XhoI | TAATAACTCGAGCAGATAAAGTGCTGAAGAGGC |
| F-NAT-BglII | TAATAAAGATCTGCTGCGAGGATGTGAGCTGG |
| R-NAT-MunI | TAATAACAATTGAAGCTTATAGAAGAGATGTAGAAACTAGC |
| F-GIS2upKO-XbaI | TAATAATCTAGAGGGCATCAACAAAGTTTGC |
| R-GIS2upKO-BglII | TAATAAAGATCTCTCAGAAAGCAAGTGGGTGG |
| F-GIS2downKO-MunI | TAATAACAATTGTCGTTGTTGGATTGTAAGCG |
| R-GIS2downKO-XhoI | TAATAACTCGAGGAGAACAGCAAGAGCGACG |
| F-NEO-BglII | TAATAAAGATCTCAGGATTCGAGTGGCATGG |
| R-NEO-BglII | TAATAACAATTGCGACGGCCAGTGAATTGTAATACG |
| F-GIS2cDNA-BamHI | TAATAAGGATCCGATGTTCGGTGCTCCTCGAGG |
| R-GIS2cDNA-BamHI | TAATAAGGATCCTTAGGCAGCAGGGGCTTCAGC |
| F-GIS2complement | TGCAGGATGAGGAGACAGC |
| R-GIS2complement | GATGACCACGGTGTGATCG |
| F-ERG25-probe | TCGACAAGTACATCCCCGG |
| R-ERG25-probe | CGTTCTTTCCCCGCTTGCC |
| RNA oligonucleotides | |
| TYE705-RPL2-3′UTR | 5′-TYE705-UGCAGUGGAGUUGGAGUGGGGAUGGGAUGUUGGGCAGUGGGCCCGUGGAU |
| Unlabeled competitor | UGCAGUGGAGUUGGAGUGGGGAUGGGAUGUUGGGCAGUGGGCCCGUGGAU |
| mt competitor | UGCAGUGGAGUUGGAGUGAAACCAAACCGUUGGGCAGUGGGCCCGUGGAU |
| Biotin-RPL2-3′UTR | 5′-biotin-UGCAGUGGAGUUGGAGUGGGGAUGGGAUGUUGGGCAGUGGGCCCGUGGAU |
| Biotin-RPL2-3′UTRmt | 5′-biotin-UGCAGUGGAGUUGGAGUGAAACCAAACCGUUGGGCAGUGGGCCCGUGGAU |
a
The mutation introduced into the RNA oligonucleotide is shown underlined.