FIG 1 .

NanoString detection method schematic. Frozen samples are lyophilized, and total RNA is extracted. Total RNA is mixed with the probe sets, with each containing a reporter and capture probe for each target RNA. After hybridization, the excess probes are removed and the probe-target complexes are aligned and immobilized in the nCounter cartridge. Fluorescent color sequences are counted and tabulated, yielding counts that correspond to the number of target molecules.