Specificity of select bacterial rRNA targeting probe sets. Control mixes were prepared with total RNA from three to five species per mix and analyzed using all of the probe sets listed on the x axis. Strain details are shown in Table S2. The probe sets for taxa not present in any of the RNA mixes (in gray font) yielded no counts above background, indicating no cross-reaction with RNAs in the mixes. All data are presented as counts, and all taxon abbreviations are defined in Table S1. The species detected by each probe set is defined by the first letters of the genus and species name, except for Streptococcus-targeting probe sets, which are abbreviated “St.” to differentiate them from Staphylococcus-targeting probe sets, which are abbreviated “S.” Probe sets that detect multiple species within a genus are indicated by “sp” as for the probe sets that detect Staphyloccocus species (S.sp), Streptococcus species (St.sp), and Burkholderia spp. (B.sp).