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. 2001 Oct 30;98(23):13120–13125. doi: 10.1073/pnas.231115398

Figure 2.

Figure 2

Meis1a does not alter Hoxa9 nuclear localization in Hoxa9-immortalized myeloid progenitors. Localization of Hoxa9 in Hoxa9HF1 cells (A–C) or Hoxa9HF1 cells expressing Meis1a (D–F), by using deconvolution microscopy and anti-Hoxa9 sera (green) (A, C, D, and F) and Hoescht 33258 (blue) as a nuclear stain (B, C, E, and F). (C and F) Merged images of anti-Hoxa9 plus Hoescht dye staining. (Inset) Hoxa9-immortalized cells stained with control rabbit Ig and Hoescht dye, demonstrating that cytosolic speckles arise from nonspecific staining.