Figure 2.

Phenotypic profiles of compounds with different mechanisms of action in E. coli cells. Fluorescent imaging: (A) top: N. C. control; middle: cisplatin; bottom: compound 1 with light; (B) top: rifampicin; middle: tetracycline; bottom: nalidixic acid. The merge is the combination of the Hoechst and FM4–64 membrane stain. Colony forming experiment with various compounds: (C) left: N. C. control; middle: cisplatin; right: compound 1 with light; (D) left: nalidixic acid; middle: rifampicin; right: tetracycline. Cells were treated with each compound at MIC for 6 h before imaging or colony forming. (E) Quantitative and qualitative analysis of E. coli filamentous growth and nucleoid morphology phenotypes in response to compound treatment.