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. 2018 Jun 4;15(8):3404–3416. doi: 10.1021/acs.molpharmaceut.8b00407

Figure 4.

Figure 4

Compound 1 induces apoptosis in HL60 cells without cell cycle arrest. (A) Flow cytometry by PI/Annexin V in HL60 cells; red = apoptotic cells, blue = necrotic cells, black = dead cells. (B) Flow cytometry by PI in HL60 cells; black = G1; red = G2; white = S phase. (C) Immunoblotting of cleaved PARP and cleaved caspase 3 in HL60 cells. GAPDH was blotted as loading control. (D) Agarose gel electrophoresis of DNA laddering. HL60 cells were treated for 24 h for flow cytometry. All panels: compound 1, 20 μM; cisplatin, 20 μM; doxorubicin, 1 μM.