Table 2. Cellular Metal Uptake and Metal Content with Different Nucleic Acids Measured by AAS.
E.
coli |
HL60 |
|||||
---|---|---|---|---|---|---|
cellular uptakea | DNA nt/mcb | RNA nt/mcc | cellular uptakea | DNA nt/mcb | RNA nt/mcc | |
1 light | 10% | 2000 ± 200 | 3800 ± 600 | 0.64% | 4800 ± 400 | 5000 ± 700 |
1 dark | 2% | -d | -d | 0.11% | -d | -d |
cisplatin | 6% | 3000 ± 200 | 4700 ± 900 | 0.72% | 7000 ± 200 | 7800 ± 700 |
Cellular uptake was calculated as metal content measured in cells divided by total metal content in both cell samples and cell culture media samples.
DNA nt/mc was calculated as DNA nucleotide bases (μmol) divided by metal content measured in DNA sample (μmol).
RNA nt/mc was calculated as RNA nucleotide bases (μmol) divided by metal content measured in DNA sample (μmol).
Ruthenium levels in DNA and RNA samples were under the detection limit (<2 ppb).