Figure 5.

Ang II has no direct influence on sensory neuron membrane potential and excitability. A, B, Ang II (1 μm; 5 min) fails to influence AP firing to ramp current injection and the membrane potential of cultured mouse (A) and human (B) DRG neurons. Data are presented as mean ± SEM. ns, Not significant versus respective vehicle groups, one-way ANOVA with Bonferroni's post hoc test. C, D, Representative AP firing traces to step current injections and graphs for step current thresholds and AP frequency of cultured mouse (C) and human (D) DRG neurons are also unaffected by Ang II exposure (1 μm, 5 min). Data are presented as mean ± SEM. Numbers shown inside each column or on line graphs in all panels indicate the number of DRG neurons in ≥4 culture batches from ≥4 mice/group or ≥4 human DRG culture batches/group. ns, Not significant versus respective vehicle groups, one-way ANOVA with Bonferroni's post hoc test. AP firing traces for vehicle- and Ang II-treated conditions in all panels are offset for distinct visualization from their respective control traces.