Figure 9.

AT2R is expressed in skin MΦs, but not in the neurons microglia/MΦs, of DRGs after hindpaw Ang II injection. A, Representative confocal microscopy images of Agtr2^GFP mouse hindpaw plantar punch tissue sections showing hindpaw Ang II injection (100 pmol, ipl; 5 h after injection) enhances MΦ density (F4/80 and CD68 staining), which overlaps with GFP signal (green), suggesting AT2R expression therein. Scale bar, 50 μm. Images on the bottom rows represent magnified red dotted rectangular boxes in the respective top row images. B, Representative confocal microscopy images of Agtr2^GFP mouse DRG (L2–L5) tissue sections showing hindpaw Ang II injection (100 pmol, ipl; 5 h after injection) does not enhance microglia/MΦ density (red, Iba1 staining) in the ipsilateral DRGs, as well as no detectable GFP signal (green, with anti-GFP antibody staining), suggesting no AT2R expression in neurons and microglia/MΦ. Scale bar, 50 μm. C, Representative microscopy images of cultured-dissociated DRGs from Agtr2^GFP mice showing no detectable GFP signal (green, with anti-GFP antibody staining), suggesting no AT2R expression in neurons (red, β3-tubulin staining) and microglia/MΦ (red, Iba1 staining). Scale bar, 25 μm. D, Representative microscopy images of cultured peritoneal monocytes/MΦs from Agtr2^GFP mice showing detection of GFP signal (green, with anti-GFP antibody staining) in Iba1-stained (red) MΦs, suggesting AT2R expression. Scale bar, 25 μm.