Table 5.

Oligonucleotides used in RT-PCR experiments

Species and gene	Primer sets	Annealing temperature (°C)	Amplicon size (bp)
Mouse Agtr1a	Forward: 5′ CTGAAGCCAGTACCAGCTCT 3′	54.0	245
	Reverse: 5′ CTGGGGCAGTCATCTTGAAT 3′
Human AGTR1	Forward: 5′ GGCCAGTGTTTTTCTTTTGAATTTAGCAC 3′	55.0	202
	Reverse: 5′ TGAACAATAGCCAGGTATCGATCAATGC 3′
Mouse/human Agtr2/AGTR2	Forward: 5′ GTTCCCCTTGTTTGGTGTAT 3′	51.0	274
	Reverse: 5′ CATCTTCAGGACTTGGTCAC 3′
Mouse/human Gapdh/GAPDH	Forward: 5′ TGATGACATCAAGAAGGTGGTGAAG 3′	57.0	240
	Reverse: 5′ TCCTTGGAGGCCATGTAGGCCAT 3′
Mouse Ly6g	Forward: 5′ CTTCTCTGATGGATTTTGCGTTG 3′	52	365
	Reverse: 5′ CAACTCAGAGCTTCTATATCTC 3′
Mouse/human Trpv1/TRPV1	Forward: 5′ CTGGACTACCTGGAACACCA 3′	53.0	128
	Reverse: 5′ AACCAGGGCAAAGTTCTTCC 3′