Figure 8.

The effects of gastrodin (GAS) preconditioning inhibited mPTP opening in H9c2 cells induced by A/R. (a) The opening of mPTP was reflected by absorbance at a wavelength of 520 nm after adding 200 mM CaCl₂ and monitoring over 20 minutes. (b) Changes in the absorbance value at 520 nm/min (∆OD·min⁻¹) were used to express the level of mPTP opening (∆OD = A520_{0 min} − A520_{20 min}). Data are expressed as the mean ± SEM (n = 3). ^∗∗P < 0.01 versus control group, ^##P < 0.01 versus A/R group, and ^▲▲P < 0.01 versus GAS + A/R group.