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. 2018 Aug 8;19:55. doi: 10.1186/s12863-018-0660-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Degenerated myofilaments and mitochondrial breakdown in the muscle of aged mice. Ultrastructure of the rectus femoris from male mice (a) at the ages of 3 months and (b-c) 24 months old. b Aged rectus femoris muscle displayed large tubular aggregates, formed by SR. c The MVB was enlarged and shown in the aged skeletal muscle. The red arrow indicates the MVB. N, nucleus; m, mitochondria; mf, myofilament; SR, sarcoplasmic reticulum. We performed ultrastructural observation using 4 mice of each age, 5 blocks of each mouse, and 3 grids of each block. We screened each grid and collected more than 5 images for us to show the appropriate and reproducible data