Fig. 3.

Effect of pre-induction Kar2/Bip content on methanol induced IP production and post-induction Kar2/Bip level. Cells were first grown in the 10 L bioreactor in a batch procedure with 95 g/L glycerol as carbon source. Subsequently, they were subjected to methanol-induced IP production in shake flask fed-batch cultures starting either with cells from the exponential, declining, or stationary growth phase of the batch culture. a Methanol-induced secretory IP production and b relative intra- and extracellular pre- and post-induction Kar2/Bip abundance. c Comparative immunoblots probing for intra- and extracellular Kar2/Bip from samples of IP producing and host cells taken from bioreactor glycerol batch and shake flask methanol fed-batch cultures. The bands inside the highlighted rectangles indicate the intracellular Kar2/Bip level prior to methanol addition