Table 1.
Equipment | Features | |||
---|---|---|---|---|
advantages | disadvantages | |||
Traditional Methods | Ultracentrifugation | Ultracentrifuge | High sample capacity; Protein and RNA components are not affected; facilitating later research |
Time-consuming; instrument-dependent; low purity |
Density gradient | Ultracentrifuge | High separation efficiency; high purity; won't to be crushed or deformed |
Long run time; equipment dependence; low yield; complex process |
|
Immunomagnetic Beads | Magnetic bead, antibody | Save time; Maintain integrity; Convenient operation; Not affected by exosome size; No need for expensive instruments |
High reagent cost; low capacity and low yields | |
Exoquick™ | Exoquick™ | Simple steps, quick operation; size uniformity; suitable for small samples, such as serum | Impurity; Affected by exosome diameter; expensive reagents; low production | |
Chromatography | Gel filtration column | High purity; uniform in size | Low extraction volume; extensive laboratory equipment requirements | |
| ||||
Novel Methods | Stirred ultrafiltration | Ultrafiltration membrane, nitrogen | Do not rely on equipment; less time consuming; Reduces the destruction of exosomes during the process | Moderate purity of isolated exosomes; loss of exosomes during the process |
Filtration Device | Microfluidic device | Fast, low cost; easy automation and integration; high portability | Lack of standardization and large scale tests on clinical samples, lack of method validation; low sample capacity | |
nPES | GNPs, antibodies | Fast, efficient, high purity; quantitative analysis | High reagent cost; complex statistical tools; low capacity | |
Membrane modification | Magnetic field, magnetic nanoparticles | Need not antibodies; save time; preserve the original structure of the exosomes; drug carriers | Complicated operation; impurity | |
ExoTIC | ExoTIC, syringe pump | Simple operation, exosome in a specific range, high purity | Special equipment requirements; Lack of tests on clinical samples, |