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GDP/GTP exchange assays. HeLa cell extracts expressing GEF1 or GEF2 of UNC-73 were assayed for their ability to catalyze GDP release from RhoA and Rac1, as described in Materials and Methods. (A) Relative amounts of bound [3H]-GDP after incubation of GEF1 or GEF2 with Rac1 or RhoA. (B) Schematic diagram of UNC-73.
