(a).

	Kato et al., 1998 [19]	Xiaoguang et al., 1998 [20]	Dai et al., 2001 [21]	Nam et al., 2003 [22]	Baral and Chattopadhyay, 2004 [23]	Leyon and Kuttan, 2004 [24]	Yoo et al., 2004 [25]	Duong Von Huyen et al., 2006 [26]	Jiménez-Medina et al., 2006 [27]	Sheeja et al., 2006 [28]	Sunila and Kutan, 2006 [29]	Xu et al., 2006 [30]	Agrawal and Jain, 2009 [31]	Agrawal and Pandey, 2009 [32]	Harhaji Trajković et al., 2009 [33]	Kwon et al., 2009 [34]	Youn et al., 2009 [35]	Agrawal and Beohar, 2010 [36]
Title
Accurate and concise description of the content of the article	✓		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓		✓	✓
Abstract
Summary of the background, objectives, methods, principal findings, and conclusions	✓		✓						✓	✓				✓	✓	✓
Introduction
Background	✓		✓	✓	✓	✓	✓		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Objectives	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Materials and methods
Ethical statement
Indicate the nature of the ethical review permissions and relevant licenses			✓			✓		✓	✓	✓	✓		✓	✓	✓	✓	✓	✓
Study design
Number of experimental and control groups	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Any steps taken to minimize the effects of subjective bias when allocating animals to treatment	✓		✓						✓			✓		✓	✓	✓		✓
The experimental unit	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Experimental procedures
Doses	✓	✓	✓	✓	✓	✓	✓		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Method of administration	✓	✓	✓	✓	✓	✓		✓	✓	✓		✓	✓	✓	✓	✓	✓	✓
Choose of dose	✓		✓							✓
Experimental animals
Origin of animal	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Species	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Sex		✓		✓		✓					✓	✓	✓	✓		✓	✓	✓
Developmental stage	✓		✓		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓
Weight		✓		✓					✓	✓	✓	✓	✓	✓	✓	✓		✓
Housing and husbandry
Housing				✓	✓	✓			✓		✓		✓	✓		✓	✓
Husbandry conditions				✓	✓	✓	✓	✓	✓	✓	✓		✓	✓	✓	✓		✓
Sample size
Specify the total number of animals used in each experiment	✓	✓	✓	✓		✓	✓	✓	✓		✓	✓	✓	✓	✓	✓		✓
Explain how the number of animals was decided
Allocating animals to experimental groups
How animals were allocated to experimental group (AZAR)	✓		✓						✓			✓		✓	✓	✓		✓
Experimental outcomes
Clearly define the primary and secondary experimental outcomes assessed	✓	✓	✓	✓		✓	✓	✓	✓	✓	✓	✓		✓	✓	✓	✓
Statistical methods
Provide details of the statistical methods used for each analysis	✓		✓			✓	✓	✓		✓	✓	✓	✓	✓	✓	✓	✓	✓
Specify the unit of analysis for each dataset	✓		✓		✓	✓	✓	✓		✓	✓	✓			✓	✓	✓	✓
Describe any methods used to assess whether the data met the assumptions of the statistical approach					✓			✓		✓
Results
Baseline data
For each experimental group, report relevant characteristics and health status of animals before treatment or testing														✓
Numbers analyzed
Report the number of animals in each group included in each analysis	✓	✓	✓	✓				✓	✓		✓				✓			✓
If any animals or data were not included in the analysis, explain why	✓	✓
Outcomes and estimation
Report the results for each analysis carried out	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓			✓	✓	✓	✓
Adverse events
Give details of all important adverse events in each experimental group			✓	✓											✓
Describe any modifications to the experimental protocols made to reduce adverse events				✓
Discussion
Interpretation/scientific implications
Interpret the results, taking into account the study objectives and hypotheses, current theory, and other relevant studies in the literature	✓		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓			✓	✓	✓
Comment on the study limitations including any potential sources of bias				✓													✓
Generalizability/translation
Comment on whether, and how, the findings of this study are likely to translate to other species or systems, including any relevance to human biology															✓
Funding
List all funding sources	✓		✓	✓				✓	✓					✓	✓	✓	✓	✓
Results (%)	65.7	40.0	68.6	62.9	51.4	57.1	45.7	54.3	65.7	57.1	60.0	57.1	51.4	65.7	74.3	68.6	57.1	62.9