Figure 2.

Identification of the Different Neural Cell Types Transduced by Peripherally Administered Anc80L65, AAV9, and scAAV9 Harboring a Self-Complementary Genome

Double immunostainings were performed in order to determine the main neural cell types transduced by each vector. Colocalization between EGFP and NeuN (neuronal nuclei) (A), or EGFP and GS (glutamine synthetase) (B) suggest that neurons and astrocytes are mostly targeted by Anc80L65, AAV9, and scAAV9 delivered intravenously. (C) In the corpus callosum, few oligodendrocytes (identified by the marker Olig2) expressing EGFP were also detected. Higher magnification images of individual cells are presented on the right panels for each cell type. By contrast, no transduced microglial cells (Figure S4) were observed. Scale bar: 50 μm.