Figure 3.

Neuronal Sub-types Targeted by Anc80L65 after Peripheral Injection

Representative images of co-labeling experiments performed to characterize the neuronal sub-types transduced by Anc80L65 (double-positive cells are indicated by white arrows). The colocalization between EGFP with CaMKII (A) or GAD67 (B) markers demonstrates efficient targeting of excitatory and inhibitory neurons, respectively. (C) Interestingly, EGFP/tyrosine hydroxylase (TH)-positive dopaminergic neurons were also observed in the substantia nigra. (D) In the cerebellum, numerous Purkinje cells, identified with the marker Calbindin, express EGFP. (E) In the spinal cord, EGFP-positive motor neurons expressing the choline acetyltransferase (ChAT) were observed. Scale bar: 50 μm.