TABLE 2 .
Strain | Recipient strain |
Transformation substrate | MIC (µg/ml)a |
Mosaic mtrDb |
Mosaic mtrpb |
|||
---|---|---|---|---|---|---|---|---|
AZI | CV | PB | TX-100 | |||||
Genomic DNA (gDNA) transformant strains | ||||||||
28BlΔGCGS0276-gDNA | 28Bl | GCGS0276 gDNA | 2 | 16 | 500 | >16,000 | + | + |
GCGS0353ΔGCGS0276-gDNA | GCGS0353 | GCGS0276 gDNA | 1 | 16 | 1,000 | >16,000 | + | + |
GCGS0465ΔGCGS0276-gDNA | GCGS0465 | GCGS0276 gDNA | 2 | 16 | 500 | >16,000 | + | + |
28BlΔGCGS0402-gDNA | 28Bl | GCGS0402 gDNA | 2 | 16 | 500 | >16,000 | + | + |
GCGS0353ΔGCGS0402-gDNA | GCGS0353 | GCGS0402 gDNA | 2 | 16 | 500 | >16,000 | + | + |
GCGS0465ΔGCGS0402-gDNA | GCGS0465 | GCGS0402 gDNA | 2 | 16 | 500 | >16,000 | + | + |
28BlΔGCGS0834-gDNA | 28Bl | GCGS0834 gDNA | 2 | 16 | 500 | >16,000 | + | + |
GCGS0353ΔGCGS0834-gDNA | GCGS0353 | GCGS0834 gDNA | 2 | 16 | 500 | >16,000 | + | + |
GCGS0465ΔGCGS0834-gDNA | GCGS0465 | GCGS0834 gDNA | 2 | 16 | 500 | >16,000 | + | + |
PCR product transformant strains | ||||||||
28BlΔGCGS0276-mtrRpCDE | 28Bl | GCGS0276 mtrRpCDE | 1 | 16 | 500 | >16,000 | + | + |
28BlΔGCGS0276-mtrD | 28Bl | GCGS0276 partial mtrC (1185–1241 bp) and mtrD (1–3174 bp) |
0.5 | 8 | 250 | >16,000 | + | − |
28BlΔGCGS0276-mtrD/18-3174 | 28Bl | GCGS0276 partial mtrD (18–3174 bp) | 0.5 | 8 | 250 | 16,000 | + | − |
28BlΔGCGS0276-mtrD/+262-2724 | 28Bl | GCGS0276 partial mtrC (1185–1241 bp) and mtrD (1–2724 bp) |
0.5 | 8 | 250 | >16,000 | + | − |
28BlΔGCGS0276-mtrDCp | 28Bl | GCGS0276 mtrD, mtrC, mtr promoter | 1 | 16 | 500 | >16,000 | + | + |
28BlΔGCGS0402-mtrRpCDE | 28Bl | GCGS0402 mtrRpCDE | 2 | 16 | 500 | >16,000 | + | + |
28BlΔGCGS0402-mtrDCp | 28Bl | GCGS0402 mtrD, mtrC, mtr promoter | 2 | 16 | 500 | >16,000 | + | + |
AZI, azithromycin; CV, crystal violet; PB, polymyxin B; TX-100, Triton X-100. MIC values are the modes from three independent replicate tests.
The presence (+) of mosaic alleles was screened for by Sanger or whole-genome sequencing.