TABLE 2 .

MIC values of transformant strains

Strain	Recipient strain	Transformation substrate	MIC (µg/ml)a				Mosaic mtrDb	Mosaic mtrpb
			AZI	CV	PB	TX-100
Genomic DNA (gDNA) transformant strains
28BlΔGCGS0276-gDNA	28Bl	GCGS0276 gDNA	2	16	500	>16,000	+	+
GCGS0353ΔGCGS0276-gDNA	GCGS0353	GCGS0276 gDNA	1	16	1,000	>16,000	+	+
GCGS0465ΔGCGS0276-gDNA	GCGS0465	GCGS0276 gDNA	2	16	500	>16,000	+	+
28BlΔGCGS0402-gDNA	28Bl	GCGS0402 gDNA	2	16	500	>16,000	+	+
GCGS0353ΔGCGS0402-gDNA	GCGS0353	GCGS0402 gDNA	2	16	500	>16,000	+	+
GCGS0465ΔGCGS0402-gDNA	GCGS0465	GCGS0402 gDNA	2	16	500	>16,000	+	+
28BlΔGCGS0834-gDNA	28Bl	GCGS0834 gDNA	2	16	500	>16,000	+	+
GCGS0353ΔGCGS0834-gDNA	GCGS0353	GCGS0834 gDNA	2	16	500	>16,000	+	+
GCGS0465ΔGCGS0834-gDNA	GCGS0465	GCGS0834 gDNA	2	16	500	>16,000	+	+
PCR product transformant strains
28BlΔGCGS0276-mtrRpCDE	28Bl	GCGS0276 mtrRpCDE	1	16	500	>16,000	+	+
28BlΔGCGS0276-mtrD	28Bl	GCGS0276 partial mtrC (1185–1241 bp) and mtrD (1–3174 bp)	0.5	8	250	>16,000	+	−
28BlΔGCGS0276-mtrD/18-3174	28Bl	GCGS0276 partial mtrD (18–3174 bp)	0.5	8	250	16,000	+	−
28BlΔGCGS0276-mtrD/+262-2724	28Bl	GCGS0276 partial mtrC (1185–1241 bp) and mtrD (1–2724 bp)	0.5	8	250	>16,000	+	−
28BlΔGCGS0276-mtrDCp	28Bl	GCGS0276 mtrD, mtrC, mtr promoter	1	16	500	>16,000	+	+
28BlΔGCGS0402-mtrRpCDE	28Bl	GCGS0402 mtrRpCDE	2	16	500	>16,000	+	+
28BlΔGCGS0402-mtrDCp	28Bl	GCGS0402 mtrD, mtrC, mtr promoter	2	16	500	>16,000	+	+

^aAZI, azithromycin; CV, crystal violet; PB, polymyxin B; TX-100, Triton X-100. MIC values are the modes from three independent replicate tests.

^bThe presence (+) of mosaic alleles was screened for by Sanger or whole-genome sequencing.