TABLE 1 .

Bacterial strains and plasmids used in this work

Strain or plasmid	Descriptiona	Source or reference
Strains
E. coli DH5α	F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoP recA1 endA1 hsdR17 (rK− mK−)	Invitrogen
E. coli S17-1λpir	recA thi pro hsdR hsdM+ RP4-2-Tc::Mu::Km Tn7 λpir (lysogen); Tpr Strepr	83
K. pneumoniae KPPR1	Rifr derivative of ATCC 43816	77
K. pneumoniae KPPR1S	Strepr derivative of KPPR1	31
K. pneumoniae VK060	KPPR1 manC::Tn5:km	77
K. pneumoniae VK279	KPPR1S 0496::pKAS46	This work
K. pneumoniae VK280	KPPR1S 4504::pKAS46	This work
K. pneumoniae VK277	KPPR1S ΔkvrA (VK055_0496)	This work
K. pneumoniae VK410	KPPR1S ΔkvrB (VK055_4504)	This work
K. pneumoniae VK332	KPPR1S ΔmarR (VK055_0870)	This work
K. pneumoniae VK278	KPPR1S kvrA complemented at native site	This work
K. pneumoniae VK417	KPPR1S kvrB complemented at native site	This work
K. pneumoniae VK322	KPPR1S 4417::pKAS46	This work
K. pneumoniae VK323	KPPR1S 4238::pKAS46	This work
K. pneumoniae VK324	KPPR1S 1202::pKAS46	This work
K. pneumoniae VK325	KPPR1S 2679::pKAS46	This work
K. pneumoniae VK326	KPPR1S 4504::pKAS46	This work
K. pneumoniae VK327	KPPR1S 1021::pKAS46	This work
K. pneumoniae VK328	KPPR1S 1682::pKAS46	This work
K. pneumoniae NTUH-K2044	Wild-type K. pneumoniae from liver abscess	47
K. pneumoniae VK559	NTUH-K2044 p0496::pKAS46	This work
K. pneumoniae K560	NTUH-K2044 p4504::pKAS46	This work
K. pneumoniae MP103	Carbapenem-sensitive derivative of KPNIH1	50
K. pneumoniae VK02	MKP103 kvrA mutant (KPNIH1_14625)	50
K. pneumoniae VK44	MKP103 kvrB mutant (KPNIH1_20355)	50
Plasmids
pKAS46	Kanr, oriR6K cloning vector	78
p0496::pKAS46	pKAS46 with an internal fragment from VK055_0496	This work
p4417::pKAS46	pKAS46 with an internal fragment from VK055_4417	This work
p4238::pKAS46	pKAS46 with an internal fragment from VK055_4238	This work
p1202::pKAS46	pKAS46 with an internal fragment from VK055_1202	This work
p2679::pKAS46	pKAS46 with an internal fragment from VK055_2679	This work
p4504::pKAS46	pKAS46 with an internal fragment from VK055_4504	This work
p1021::pKAS46	pKAS46 with an internal fragment from VK055_1021	This work
p1682::pKAS46	pKAS46 with an internal fragment from VK055_1682	This work
pKAS46ΔkvrA	kvrA flanking regions in pKAS46	This work
pKAS46ΔkvrB	kvrB flanking regions in pKAS46	This work
pKAS46 kvrAcomp	kvrA flanking regions and gene in pKAS46	This work
pKAS46 kvrBcomp	kvrB flanking regions and gene in pKAS46	This work
pPROBE_tagless	Kanr, gfp reporter vector	79
pPROBE_galF	galF promoter of KPPR1S in pPROBE	This work
pPROBE_wzi	wzi promoter of KPPR1S in pPROBE	This work
pPROBE_manC	cpsB promoter of KPPR1S in pPROBE	This work

^aCm^r, chloramphenicol resistance; Tp^r, trimethoprim resistance.