TABLE 2 .
Primers used in this study
| Primer | Sequencea (5′ to 3′) | Descriptionb |
|---|---|---|
| MP159 | TGACTAGATATCCGACACTCTTAACCAACAGCT | Δ0496 5′ flank F |
| MP160 | TGCATATCTAGACTCCAGGCTAAAGATTAATTC | Δ0496 5′ flank R |
| MP161 | TGCATATCTAGATGGCGATTCCAATTTCATCTC | Δ0496 3′ flank F |
| MP162 | TCGATAGCGGCCGCCATGCGGCAATCAGGGCGACG | Δ0496 3′ flank R |
| MP228 | TGCATATCTAGAGTGCGCACACCTATAAGCGTA | manC promoter F |
| MP229 | CAGTACGAATTCGCTCGCGAGACATCGGCCAGA | manC promoter R |
| MP232 | TGCATATCTAGACGGTAATTGATAATTCATATT | wzi promoter F |
| MP233 | CAGTACGAATTCTGGGCTCCCAGGGAGGAAAGC | wzi promoter R |
| MP234 | TGCATATCTAGACTGTACGACTGCGGTATGTGT | galF promoter F |
| TM2 | CAGTACGAATTCTTTGTGGCCGGCAGCATATGC | galF promoter R |
| MP251 | TGACTAGATATCCACGACACCATCAGGATGGCG | Δ0870 5′ flank F |
| MP252 | TGCATATCTAGAGCTGGTACTTTTCATAATGGT | Δ0870 5′ flank R |
| MP253 | TGCATATCTAGACTCAAGAAGGTCCTGCCGTAA | Δ0870 3′ flank F |
| MP254 | TCGATAGCGGCCGCCAAGAGTACGATAGCTGCGGC | Δ0870 3′ flank R |
| MP256 | TGACTAGATATCGGAACGGTTAAGCAACGCCTC | 4417::kan F |
| MP257 | TGCATATCTAGACTGACTAAACCTCAGTATGCG | 4417::kan R |
| MP258 | TGACTAGATATCCTCCAGCGCCTTCAGACGATG | 4238::kan F |
| MP259 | TGCATATCTAGAGCGGCCAGACCTCGACTGCAG | 4238::kan R |
| MP260 | TGACTAGATATCGCTGACGGTGGTGTAATCGCG | 1202::kan F |
| MP261 | TGCATATCTAGATCAGCGTAATGAACCAGCCGC | 1202::kan R |
| MP262 | TGACTAGATATCCGGCGGTCAGCGTCAGCAGGC | 2679::kan F |
| MP263 | TGCATATCTAGAGCCAGTGGCGCGCCATTATTG | 2679::kan R |
| MP274 | TGACTAGATATCTCAGCGATACGGGTGGCGTTG | 4504::kan F |
| MP275 | TGCATATCTAGACCGCCATGAAGATTTCCCGTT | 4504::kan R |
| MP276 | TGACTAGATATCGGCGGTCAATCGTCGGCTGAA | 1021::kan F |
| MP277 | TGCATATCTAGAGCAGTCGCTTACGTTTGTCGC | 1021::kan R |
| MP280 | TGACTAGATATCGGATCTGTTCAATAAGACCGA | 1682::kan F |
| MP281 | TGCATATCTAGAAAGAGCACGTGGAAGGGTTTC | 1682::kan R |
| MP285 | TGACTAGATATCCCGATCGAGCGTGACCAGCGG | Δ4504 5′ flank F |
| MP286 | TGCATATCTAGAGAAGGCGTGCTAGCGGACTCT | Δ4504 5′ flank R |
| MP287 | TGCATATCTAGAAAACGAACTATCCATTTGGGT | Δ4504 3′ flank F |
| MP288 | TCGATAGCGGCCGCTCTGCGGGTGTCTGACGGCGC | Δ4504 3′ flank R |
| KW372 | TAATCGACCATCGCCTGAAAC | kvrA 5′ flank F |
| KW373 | GACGGCTGTTCAATACCGATAG | kvrA 3′ flank R |
| KW374 | GCCATGAAGATTTCCCGTTTC | kvrB 5′ flank F |
| KW375 | CGTTAATTCCCTGCGCTTTG | kvrB 3′ flank R |
a
Restriction sites are in bold.
b
F, forward primer; R, reverse primer.