TABLE 1 .
Strains and plasmids used in this study
| Strain, plasmid, or cell line |
Description | Reference or source |
|---|---|---|
| Bacterial strains | ||
| WAM2266 | CFT073 Nal; urosepsis isolate | 18 |
| WAM5009 | CFT073 with pFUSE ipuR txn fusion | This study |
| WAM5063 | CFT073 Nal fimBE fimX ipuA ipuB (fimS OFF, ipuS OFF) | This study |
| WAM5064 | CFT073 Nal fimBE fimX ipuA ipuB (fimS ON, ipuS ON) | This study |
| WAM5065 | CFT073 Nal fimBE fimX ipuA ipuB (fimS ON, ipuS OFF) | This study |
| WAM5088 | CFT073 Nal fimBE fimX ipuA ipuB (fimS OFF, ipuS ON) | This study |
| WAM5144 | WAM5063 lacZYA | This study |
| WAM5145 | WAM5064 lacZYA | This study |
| WAM5146 | WAM5065 lacZYA | This study |
| WAM5147 | WAM5088 lacZYA | This study |
| WAM5070 | WAM5064 with pACYC184 | This study |
| WAM5079 | WAM5064 with pACYC177 | This study |
| WAM5071 | WAM5064 with pWAM2801 | This study |
| WAM5081 | WAM5064 with pWAM2957 | This study |
| WAM5072 | WAM5064 with pWAM2961 | This study |
| WAM5080 | WAM5064 with pWAM2775 | This study |
| WAM5082 | WAM5064 with pWAM3579 | This study |
| WAM5073 | WAM5064 with pWAM5073 | This study |
| WAM5074 | WAM5065 with pACYC184 | This study |
| WAM5083 | WAM5065 with pACYC177 | This study |
| WAM5075 | WAM5065 with pWAM2801 | This study |
| WAM5085 | WAM5065 with pWAM2957 | This study |
| WAM5076 | WAM5065 with pWAM2961 | This study |
| WAM5084 | WAM5065 with pWAM2775 | This study |
| WAM5077 | WAM5065 with pWAM3759 | This study |
| WAM5078 | WAM5065 with pWAM5073 | This study |
| MS427 | E. coli expression strain | 48 |
| MS427(pUpaE) | MS427 with pSU2718::upaE | This study |
| Plasmids | ||
| pKD46 | Lambda Red recombinase helper plasmid | 92 |
| pCP20 | FLP helper plasmid | 92 |
| pKD4 | kan+ template | 92 |
| p2779 | pFUSE lacZYA transcriptional fusion suicide vector | 93 |
| pACYC177 | Cloning plasmid with constitutively active promoter | New England BioLabs |
| pACYC184 | Cloning plasmid with expression driven by the insert promoter | New England BioLabs |
| pMal-p2x | Cloning plasmid for MBP-assisted protein purification | New England BioLabs |
| pSU2718 | Low-copy-number cloning plasmid for exogenous expression in E. coli | 46 |
| pWAM2801 | pACYC177 fimB; Kanr | 23 |
| pWAM2957 | pACYC177 fimE; Kanr | 23 |
| pWAM2775 | pACYC177 ipuB; Kanr | 23 |
| pWAM2961 | pACYC177 ipuA-FL; Kanr | 23 |
| pWAM3579 | pACYC184 ipuA-FL; Cmr | 23 |
| pWAM5073 | pACYC184 ipuA-Trunc; Cmr | This study |
| pUpaE | pSU2718 upaE | This study |
| Cell line A-498 | Human kidney epithelial carcinoma cells | ATCC |