FIG 1 .

Deletion of yhdP causes SDS EDTA and vancomycin sensitivity. (A) Cells with wild-type yhdP or from a ΔyhdP deletion mutant were diluted into fresh media containing 0.05% SDS and the indicated concentration of EDTA, and growth was assayed based on the OD₆₀₀ every 10 min. The ΔyhdP strain was more sensitive to EDTA in the presence of SDS than the yhdP⁺ strain. (B) Cells were grown as described for panel A, with the indicated concentration of vancomycin. The ΔyhdP strain lysed at lower concentrations of vancomycin than those that affected the yhdP⁺ strain. Data are averages of three independent biological replicates ± the SEM on a log₁₀ scale.