Fig. 4.

Native MS after reduction with DTT. Native MS spectra of 10 μM CYGB in 100 mM ammonium acetate after adding 10 mM DTT. Monomeric +9 is highlighted. Inset: the zoomed in section of the monomeric +9 peak. Addition of DTT leads to a loss of the dimers via reduction of disulfide bridges. DTT also seems to reduce the number of oxidations (narrowing of the peaks). See also Fig. 2 for comparison.