Table 1.
Oxidative modifications of CYGB. A list of the number of different amino acids in CYGB (# AA in sequence) and the number of oxidized amino acids of each type before (control) and after different treatment times. Eight amino acids (M, Y, D, F, H, W, N, and P) were chosen for analysis. Samples were measured with Ion trap LC-MS/MS and spectra were analyzed with Mascot and Sequest algorithms.
|
Tris-buffer | |||||||
|---|---|---|---|---|---|---|---|
| Amino Acid | # AA in sequence | Control | 30 s | 1 min | 3 min | 5 min | 10 min |
| Methionine (M) | 6 | 2 | 3 | 4 | 4 | 3 | 4 |
| Tyrosine (Y) | 5 | 2 | 3 | 3 | 2 | 3 | 2 |
| Tryptophan (W) | 3 | 1 | 2 | 2 | 2 | 2 | |
| Phenylalanine (F) | 8 | 1 | 1 | 1 | 1 | 1 | 1 |
| Aspartic acid (D) | 5 | 1 | 2 | 2 | 1 | ||
| Asparagine (N) | 5 | 3 | 3 | 1 | 2 | ||
| Proline (P) | 13 | 1 | 3 | ||||
| Histidine (H) | 6 | 1 | 1 | 1 | 1 | ||
| TOTAL | 51 | 5 | 12 | 16 | 13 | 13 | 14 |