Fig. 4. Regulation of EGFR and STAT3 in cells 24 h post-treatment. A549 cells were fixed, stained and observed for 24 h post-treatment with compounds 6b, 6h and 6i at a 2 μM final concentration under a confocal microscope (FLOW VIEW FV 1000 series). The Cy3 dye was used to stain both EGFR and STAT3, whereas DAPI was used as a nuclear stain. Compounds 6h and 6i showed profound inhibitory effects on the levels of EGFR and STAT3.
