Figure 2.

Interaction between JP2 and SK2 in vitro. A, Schematic diagrams of mouse full‐length JP2 and three JP2 constructs. B, GST fusion proteins containing GST‐JP2 and GST‐JP2 constructs (GSTJP2‐N1, GSTJP2‐N2 and GSTJP2‐C) were performed to pull down the mouse heart lysate followed by immunoblotting with an anti‐SK2 antibody. Positive bands displayed interactions of GST‐JP2 with SK2 and GSTJP2‐N1 with SK2. No interaction was observed between SK2 and GSTJP2‐N2, GST‐JP2‐C or GST alone. C, GST fusion proteins were performed to pull down transfected HEK293 cells followed by immunoblotting with an anti‐SK2 antibody. Positive bands showed the binding of GST‐JP2 with SK2 and GST‐JP2‐N1 with SK2. No interaction was observed between SK2 and GST‐JP2‐N2, GST‐JP2‐C or GST alone