Figure 4.

JP2 increases SK2 channel surface expression in HEK293 cells. A, Western blot analysis showing the total expression of the SK2 protein from HEK293 cells cotransfected with JP2 and SK2 plasmids and with the cells transfected with the SK2 channel alone. B, The surface expression of the SK2 protein from HEK293 cells stably expressing SK2 and SK2+JP2 with cell surface biotinylation assay. Signals were corrected to total channel protein input and normalized. C, The bar graphs show no changes in the total expression of the SK2 protein (input), but the surface expression of the SK2 protein (biotinylated protein) from HEK293 cells cotransfected with JP2 and SK2 plasmids significantly increased compared with the cells transfected with the SK2 channel alone (*P < .05, n=5 per group)