Figure 3.

IFNAR signaling is required for Pam2Cys‐mediated proinflammatory cytokine production and induction of preterm birth. (A and B) WT and IFNAR^−/− mice (n = 3–7/condition) were challenged with Pam3Cys (100 μg/mouse) or Pam2Cys (100 μg/mouse) for 4 h and serum IL‐6 and TNF levels were quantified by in vivo cytokine capture assay (IVCCA). (C) Gravid WT and IFNAR^−/− mice were challenged with Pam2Cys (200 μg/mouse) on day 16 of gestation and the incidence of PTB was quantified. In vivo serum cytokine values were normalized to Pam2Cys challenge as appropriate and expressed as the percent (%) change. Student's t test, **P < 0.01, ***P < 0.001