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. 2017 May 25;90(2):79–87. doi: 10.1111/tan.13057

Figure 2.

Figure 2

Workflow for full‐length HLA gene characterisation showing the dual redundant sequencing strategy using the MiSeq and PacBio RS II platforms. A, MiSeq requires a fragmentation step owing to its inability to completely sequence molecule fragments longer than 600 bp; barcodes are attached during library preparation. B, Barcoding is carried out as a part of the polymerase chain reaction (PCR)