Figure 1.

22‐S‐HC protects against ethanol‐induced fatty liver injury in mice. After an ethanol (EtOH)‐containing diet or control diet for 3 weeks, mice were administered vehicle or 22‐S‐HC for 2 weeks. (A) GPT and GOT enzyme activities and the amount of TG and MDA in the serum or liver tissues were quantified. (B) Liver sections were stained with H&E (×200). (C, D) Total RNAs obtained from liver tissues were subjected to qRT–PCR for determination of mRNA levels of the indicated genes. (E) Amounts of MCP‐1 protein in the serum or liver tissues were quantified by ELISA; numbers represent mean ± SD (n = 6 for the pair‐fed control and 22‐S‐HC groups; n = 8 for the ethanol‐fed and ethanol‐with ‐22‐S‐HC group); *p < 0.05, **p < 0.05 and ***p < 0.001 compared with the pair‐fed control group; ^# p < 0.05, ^## p < 0.01 and ^### p < 0.001 compared with the ethanol‐fed group; comparisons between groups were performed using the Mann–Whitney U‐test