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. 2018 Jul 13;9(54):30450–30464. doi: 10.18632/oncotarget.25765

Figure 8. Expression of DNA damage response proteins in melanoma cells following treatments with BRAFi and/or Chk1i.

Figure 8

Cells were treated with PLX4032 (7.5 μM) and/or PF477736 (0.15 μM) for 6 h or 16 h. (A) A375 and A375-PLX-R cells treated with drugs for 6 h; (B) WM9 and WM9-PLX-R cells treated with drugs for 6 h; and (C) A375 and A375-PLX-R cells treated with drugs for 16 h. Western blotting analyses with respective antibodies were performed. Ratios of phosphorylated Chk1 proteins at S296, S317, S345, or S280 over total Chk1 protein were calculated and then adjusted relative to the value of lane 1 (untreated A375 or WM9 cells) as described in Figure 2. Relative ratios are the averages of two sets of images from two independent experiments.