Figure 3. Gαi3-/- female mice display increased nuclear pSer133-CREB levels in the liver.
(A) Immunoblot analysis of pSer133-CREB and CREB protein levels in nuclear extracts from livers of female wild-type vs. Gαi3-/- mice. (B) Quantitative analysis of relative protein levels (pSer133-CREB/CREB) was performed by ImageJ software. Results are expressed as mean ± s.d. of three independent experiments. Mice were sacrificed and analyzed every six hours at the indicated time points (ZT 0 to ZT 18). ATF1, activating transcription factor 1.