Figure 4. Transcript levels of the DBP target gene and cytochrome P450 family member Cyp3a11 are increased in the liver of Gα_i3^-/- female mice.

Quantitative real-time PCR analysis of rhythmic expression of Cyp3a11 and Cyp2a4/a5 genes in the liver of female (A) and male (B) Gα_i3 deficient mice vs. wild-type control animals. Mice were sacrificed and analyzed every six hours at the indicated time points (ZT 0 to ZT 18). Transcript levels of Cyp3a11 and Cyp2a4/a5 were normalized to the endogenous control Gapdh. Shown are 2^-ΔCt values. Results are expressed as mean ± s.d. of five animals analyzed per genotype and time point (^*p < 0.05; ^**p < 0.01 as compared to corresponding wild-type control animals).