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. 2018 Jul 13;9(54):30304–30323. doi: 10.18632/oncotarget.25717

Figure 4.

Figure 4

(A) Effect of PJLME on colony formation. For assessing the effect of PJLME on colony forming potential, clonogenic assay was performed. Selected cell lines were treated with PJLME (16.8 μg/mL) and vehicle control (DMSO 0.1%) for 72h and re-cultured for next seven days. Stained cells were observed & colonies were counted using phase contrast microscope (Olympus, Tokyo, Japan) equipped with digital camera. (A) Images of the colonies formed in treated and control sets. (B) Statistical analysis of the clonogenic assay.