Figure 6.

Tdrd6a Stimulates Buc Mobility in BmN4 Cells

(A) Localization of Buc-eGFP and mCherry-Tdrd6a in BmN4 cells in a single transfection (upper panel) and when they are co-transfected (middle and bottom). Co-transfected BmN4 cells displaying enlarged Buc-eGFP granules to which mCherry-Tdrd6a co-localizes (middle) or dissolved Buc-eGFP (bottom). Scale bars, 10 μm.

(B) Quantification of localization of Tdrd6a transfected 1 day after Buc.

(C) Quantification of localization of Buc transfected 1 day after Tdrd6a.

(D) Co-transfection of Dcp1-mCherry with Buc-eGFP (left) or Dcp1-eGFP with mCherry-Tdrd6a (right). Scale bars, 5 μm.

(E) FRAP recovery curves of mCherry-Tdrd6a and Buc-eGFP (with or without the presence of mCherry-Tdrd6a as indicated). Fluorescence intensity is the calculated fraction of the pre-bleach intensity and is plotted with the 95% confidence interval.

(F) FRAP recovery of Buc-eGFP plotted against increasing relative amounts of mCherry-Tdrd6a present in the bleached granule.

(G) Western blot for GFP and Tdrd6a on transfected BmN4 cell lysates and corresponding pellets.

(H) Pellets of lysates of Buc-EGFP-expressing BmN4 cells in the presence or absence of Tdrd6a as indicated.

See also Figure S6.