REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC-CD34	BD Biosciences	Cat#345804 RRID: AB_2686894
PE-CD45	BD Biosciences	Cat#555483 RRID: AB_395875
PE-CD43	BD Biosciences	Cat#560199 RRID:AB_1645655
APC-CD56	BD Biosciences	Cat#555518 RRID: AB_398601
PE-CD117	BD Biosciences	Cat#340529 RRID: AB_400044
PE-CD3	BD Biosciences	Cat#552127 RRID: AB_394342
PE-CD94	BD Biosciences	Cat#555889 RRID: AB_396201
PE-NKG2D	BD Biosciences	Cat#561815 RRID: AB_10896282
PE-NKp46	BD Biosciences	Cat#557991 RRID: AB_396974
PE-NKp44	BD Biosciences	Cat#558563 RRID: AB_647239
PE-TRAIL	BD Biosciences	Cat#565499 RRID: N/A
PE-FAS ligand	BD Biosciences	Cat#564261 RRID:N/A
PE-CD16	BD Biosciences	Cat#555407 RRID: AB_395807
APC-anti-human MSLN	R&D Systems	Cat#FAB32652A RRID: AB_2298058
PE-NKG2A	R&D Systems	Cat#FAB1059P RRID: AB_2132978
PE-goat-anti-mouse IgG F(ab)2-	Affymetrix eBioscience Inc.	Cat#15208759 RRID:N/A
APC-CD107a	Affymetrix eBioscience Inc.	Cat#14-1079-80 RRID: AB_467426
PacBlue-IFN-γ	Biolegend	Cat#502522 RRID: AB_893525
PacBlue-CD45	Biolegend	Cat#103126 RRID: AB_493535
Rabbit monoclonal anti-Fyn [EPR5500]	abcam	Cat#ab125016 RRID: AB_10972030
Rabbit monoclonal anti-Fyn (phospho Y530) + Yes (phospho Y537)	abcam	Cat# b188319 RRID:N/A
Rabbit polyclonal anti-PLCγ2	cell signaling technology	Cat#3872 RRID: AB_2299586
Rabbit polyclonal anti-phospho-PLCγ2 (Tyr1217)	cell signaling technology	Cat#3871 RRID: AB_2299548
Mouse monoclonal anti-PLCγ1 [M156]	abcam	Cat#ab41433 RRID: AB_777246
Rabbit monoclonal anti-phospho-PLCγ1 (Ser1248)	cell signaling technology	Cat#8713 RRID: AB_10890863
Rabbit monoclonal anti-Syk	cell signaling technology	Cat#2712 RRID: AB_10828107
Rabbit monoclonal anti-phospho-Syk (Tyr525/526)	cell signaling technology	Cat#2710 RRID: AB_2197222
Rabbit polyclonal anti- VAV1	abcam	Cat#ab97574 RRID: AB_10680940
Rabbit monoclonal anti-phospho-VAV1 (Y174)	abcam	Cat#ab76225 RRID: AB_1524546
Mouse monoclonal anti-p44/42 MAPK (Erk1/2) (3A7)	cell signaling technology	Cat#9107 RRID: AB_10695739
Rabbit polyclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)	cell signaling technology	Cat#9101 RRID: AB_331646
Rabbit polyclonal-anti-IKKβ	cell signaling technology	Cat#2684 RRID: AB_10699025
Rabbit monoclonal anti-phospho-IKKα/β (Ser176/180)	cell signaling technology	Cat#2697 RRID: AB_2079382
Rabbit monoclonal anti-IκBα	cell signaling technology	Cat#9242 RRID: AB_823540
Mouse monoclonal anti-phospho-IκBα (Ser32/36) (5A5)	cell signaling technology	Cat#9246 RRID: AB_2267145
Goat polyclonal-anti-NKG2D,	abcam	Cat#ab36136 RRID: AB_776794
Rabbit monoclonal anti-CD3zeta	abcam	Cat#ab40804 RRID: AB_726343
Mouse monoclonal anti-DAP10 (H-3)	santa cruz biotechnology	Cat#sc-374196 RRID: AB_10987875
Mouse monoclonal anti-GAPDH	abcam	Cat#ab8245 RRID: AB_2107448
Rabbit monoclonal anti-NCAM	abcam	Cat#ab215981 RRID:N/A
Mouse monoclonal anti-pan Cytokeratin	abcam	Cat#ab6401 RRID: AB_305450
Rabbit polyclonal anti-CD45	abcam	Cat#ab10559 RRID: AB_442811
Bacterial and Virus Strains Cat#
pKT2-mCAG-IRES-GFPZEO	Branden Moriarity lab	N/A
PiggyBac-mCAG-IRES-GFPZEO	Branden Moriarity lab	N/A
pCMV(CAT)T7-SB100	Addgene	Cat#34879
Super piggyBac Transposase expression vector	System Biosciences Inc.	Cat#PB210PA-1
pCDH-CMV-MCS-EF1α-GreenPuro	System biosciences Inc.	Cat#CD513B-1
Biological Samples
Human Serum AB	Sigma-Aldrich	Cat#H4522
Peripheral blood buffy coat	San Diego Blood Bank (https://www.sandiegobloodbank.org/)	N/A
Chemicals, Peptides, and Recombinant Proteins
α-MEM culture medium	Fisher Scientific	Cat#12634
Horse serum	Fisher Scientific	Cat#16050130
AIM V medium	Fisher Scientific	Cat# 31035025
Fetal bovine serum	Fisher Scientific	Cat# 10437010
TransDux MAX Lentivirus Transduction Enhancer	System Biosciences Inc.	Cat#LV860A-1
Chromium-51	PerkinElmer	Cat#NEZ030S001MC
Europium	PerkinElmer	Cat# AD0024
CellEvent™ Caspase-3/7 Green Detection Reagent	Thermo fisher	Cat#C10423
IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent	Essenbioscience	Cat#4440
zeomycin	Thermo Fisher	Cat#r25001
GolgiStop	BD Biosciences	Cat#554724
GolgiPlug	BD Biosciences	Cat#555029
NgoMIV	New England Biotechnology	Cat#R0564
SbfI	New England Biotechnology	Cat#R0642
Gateway LR Clonase II Enzyme mix	Thermo Fisher	Cat# 11791100
Gateway BP Clonase II Enzyme mix	Thermo Fisher	Cat#11789020
Recombinant human IL-2	Proleukin	Cat#NDC-66483-116- 07
Recombinant human IL-3	Pepro Technology Inc.	Cat#200-03
Recombinant human IL-7	R&D Systems	Cat#207-IL
Recombinant human IL-15	R&D Systems	Cat#247-IL
Recombinant human FLT-3 Ligand	Pepro Technology Inc.	Cat#300-19
Recombinant human SCF	R&D Systems	Cat#255-SC/CF
Recombinant human VEGF	R&D Systems	Cat#293-VE
Recombinant human BMP-4	R&D Systems	Cat#314-BP
Recombinant human bFGF basic	R&D Systems	Cat#4114-TC
Critical Commercial Assays
Pierce™ Classic Magnetic IP/Co-IP Kit	Thermo Fisher	Cat#88804
Dynabeads Human T-Activator CD3/CD28	Thermo Fisher	Cat#11132D
EasySep™ Human T Cell Isolation Kit	Stemcell technologies	Cat#17951
Mem-PER™ Plus Membrane Protein Extraction Kit	Thermo Fisher	Cat#89842
Human IFN-gamma Quantikine ELISA Kit	R&D Systems	Cat#SIF50
Human TNF-alpha Quantikine ELISA Kit	R&D Systems	Cat#STA00C
IL-6 Human ELISA Kit	Thermo Fisher	Cat#EH2IL6
Cell Line Optimization 4D-Nucleofector® X Kit	Lonza	Cat#V4XC-9064
P3 Primary Cell 4D-Nucleofector® X Kit L	Lonza	Cat#V4XP-3012
Experimental Models: Cell Lines
Human: iPS cells	Dan Kaufman lab	N/A
Human: NK 92 cells	ATCC	Cat#CRL-2407
Human: K-562 cells	ATCC	Cat#CCL-243
Human: MA148 cells	Sundaram Ramakrishnan lab	N/A
Human: A1847 cells	Reuben Harris lab	N/A
Human: artificial Antigen Presenting cells	Dean A. Lee lab	N/A
Experimental Models: Organisms/Strains
Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ	Jackson lab	Cat#005557
Oligonucleotides
Primer for human RNase gene (Forward :5′-GCG GAG GGA AGC TCA TCA G-3′; reverse: 5′-CTG GCC CTA GTC TCA GAC CTT-3′)	This paper	N/A
Primer for GFP:zeo (Forward :5′-ATT CTG GTT GAG CTG GAT GG-3′; reverse: 5′-GTC AGG GTG GTC ACC AGA GT-3′:	This paper	N/A
Recombinant DNA
Sequence (g-block): anti-mesothelin scFv	Chowdhury et al., 1998	N/A
gBlocks gene fragment: human CD8a extraocular domain	NCBI Reference Sequence: NM_001768	N/A
gBlocks gene fragment: human CD28 transmembrane domain	NCBI Reference Sequence: NM_006139.3	N/A
gBlocks gene fragment: human CD28 intraocular domain	NCBI Reference Sequence: NM_006139.3	N/A
gBlocks gene fragment: human CD137 intraocular domain	NCBI Reference Sequence: NM_001561.5	N/A
gBlocks gene fragment: human CD3zata intraocular domain	NCBI Reference Sequence: NM_198053.2	N/A
gBlocks gene fragment: human CD16 transmembrane domain	NCBI Reference Sequence: NM_001127593.1	N/A
gBlocks gene fragment: human NKp44 transmembrane domain	NCBI Reference Sequence: NM_001199509.1	N/A
gBlocks gene fragment: human NKp46 transmembrane domain	NCBI Reference Sequence: NM_001145457.2	N/A
gBlocks gene fragment: human NKG2D transmembrane domain	NCBI Reference Sequence: NM_007360.3	N/A
gBlocks gene fragment: human 2B4 intraocular domain	NCBI Reference Sequence: NM_001166663.1	N/A
gBlocks gene fragment: human DAP10 intraocular domain	NCBI Reference Sequence: NM_001007469.1	N/A
gBlocks gene fragment: human DAP12 intraocular domain	NCBI Reference Sequence: NM_198125.2	N/A
Software and Algorithms
Prism version7	Prism Graphpad	N/A
FlowJo	https://www.flowjo.com/	N/A
Odyssey imaging systems	IL-DOR	N/A
IncuCyte real-time image system	Essenbioscience	N/A
Xenogen IVIS imaging system	Caliper Life Science	N/A
ImageJ	https://imagej.nih.gov/ij/	N/A