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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Immunol. 2018 Jun 20;201(3):930–939. doi: 10.4049/jimmunol.1800054

Figure 3. Loss of Dicer alone is insufficient to elevate RAG1 expression in cultured BM cells.

Figure 3

(A) BM cells from mice of the indicated genotypes were cultured in the presence of IL-7 and analyzed as in Fig. 1A. Data are representative of three independent experiments. (B) Lysates prepared from 8d+IL-7 BM cultures were probed by Western blotting as in Fig. 1B. Data are representative of two independent experiments. (C) Rag1 and Rag2 transcript levels were compared between cultured BM cells from the indicated mice as in Fig. 1E. (D–E) Intracellular PTEN was detected by flow cytometry, and the mean fluorescence intensity (MFI) for PTEN staining was quantified as in Fig. 2F–G. (F) V(D)J rearrangements in sorted bone marrow pre-B cells (B220+CD43−IgM−) were analyzed by PCR and southern blotting as in Fig. 2E. Error bars represent the mean +/− SEM for three independent experiments. ns = not significant by one-way ANOVA with Bonferroni post-hoc correction.