LP1413 sequence conservation. A. Sequence of LP1413 from SCCmec type IV, with secondary structure elements colored as in Fig 1. The region included in DUF 1413 and the disordered C-terminal tail are shown below the sequence. A consensus sequence and logo generated (using Geneious (Kearse et al., 2012)) from an alignment of 113 sequences retrieved from the Uniref100 database are shown, coloured according to polarity. * underneath the logo marks residues lining the hydrophobic pocket and red * the prolines in the turn. Inset: the same cartoon as in Fig. 1C, for reference. B. Views of LP1413 monomer B oriented as in Figure 1C (left) and rotated 180°, with the sequence conservation mapped onto the surface using Consurf (Ashkenazy et al., 2016). In the left panel, M1 of monomer A is shown as white sticks, and a modelled dT as magenta sticks. See also Figure S2.